Plasmid

Part:BBa_K3887004:Design

Designed by: Ran Wang   Group: iGEM21_BJU_China   (2021-10-01)


pCas


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6015
    Illegal NotI site found at 3034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8294
    Illegal BamHI site found at 10296
    Illegal XhoI site found at 4353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10131
    Illegal AgeI site found at 11788
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 10113


Design Notes

knock out the TnaA


Source

Bluepha

References